Author: gbutler

Read the paper – https://www.nature.com/articles/s41467-024-53442-8 We describe here the construction of a large collection of gene disruptions, greatly increasing the molecular tools available for probing gene function in C. parapsilosis. We use these to identify transcription factors associated with multiple metabolic pathways, and in particular to dissect the network regulating the…

Read the paper at https://academic.oup.com/femsyr/article-lookup/doi/10.1093/femsyr/foac053 More detail at http://butler.ucd.ie/index.php/first-european-isolates-of-saccharomyces-eubayanus/ Our discovery is reported by many international newspapers and websites, including the New York Times https://www.nytimes.com/2022/12/07/science/beer-yeast-lager.html and the New Scientist https://www.newscientist.com/article/2350277-ancient-yeast-used-to-brew-the-first-lagers-discovered-in-ireland/

New publication led by Lisa Lombardi shows that editing genes with CRISPR-Cas9 in Candida parapsilosis results in Loss of Heterozygosity (LOH) near the target site. Be careful when evaluating phenotypes of mutants made using CRISPR….https://pubmed.ncbi.nlm.nih.gov/36416551/

Sean Bergin and ex-PhD student Fang Zhao led this study, in collaboration with Tobias Höhl at the Memorial Sloan Kettering Cancer Center, New York and Florent Morio at University Nantes. The paper describes the largest population study of Candida parapsilosis. In addition, we identified an amplification of a flippase gene…